Recombinant Human VIM Full Length protein, His-tagged

• Cat.No.: VIM-16H
• Product Overview: A DNA sequence encoding the human VIM ( Met 1- Glu466) was expressed, with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: 1-466 a.a.
• Description: This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 40% acetonitrile +0.1%TFA.
• Molecular Mass: The secreted recombinant human VIM consists of 476 amino acids and predicts a molecular mass of 55 KDa. The apparent molecular mass of the protein is approximately 56 KDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: VIM vimentin [ Homo sapiens (human) ]
• Official Symbol: VIM
• Synonyms: VIM; HEL113; CTRCT30; vimentin; epididymis luminal protein 113
• Gene ID: 7431
• mRNA Refseq: NM_003380
• Protein Refseq: NP_003371
• MIM: 193060
• UniProt ID: P08670
• Chromosome Location: 10p13
• Pathway: Alpha6-Beta4 Integrin Signaling Pathway; Caspase cascade in apoptosis; Epstein-Barr virus infection
• Function: double-stranded RNA binding; glycoprotein binding; protein C-terminus binding