| Cat.No. : |
VPX-897H |
| Product Overview : |
Recombinant Human VPX Protein (1-113 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length. |
| Description : |
Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription. |
| Source : |
E. coli |
| Species : |
Human |
| Tag : |
His |
| Form : |
Tris-based buffer, 50% glycerol |
| Molecular Mass : |
17.2 kDa |
| Protein length : |
1-113 aa |
| AA Sequence : |
MTDPRERVPP GNSGEETIGE AFEWLERTIE ALNREAVNHL PRELIFQVWQ RSWRYWHDEQ GMSASYTKYR YLCLMQKAIF THFKRGCTCW GEDMGREGLE DQGPPPPPPP GLV |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with concentration instruction is sent along with the products. |
| UniProt ID : |
P18099 |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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