|Product Overview :||Recombinant Human VSIR protein (Phe33-Ala194), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Platelet receptor Gi24, also known as B7-H5 and stress-induced secreted protein-1 (Sisp-1), is a protein that in humans is encoded by the C10orf54 gene, which contains 1 Ig-like (immunoglobulin-like) domain. As for C10orf54 gene, C10orf54 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state on ESCs. Human C10orf54 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment.|
|Tag :||human IgG1 Fc|
|Form :||Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 44.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to Glycosylation.|
|Protein length :||162|
|Endotoxin :||Less than 1.0 EU per ug by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of VSIR-20H :||
Human B7-H5, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||VSIR|
|Official Symbol :||VSIR|
|Synonyms :||B7H5; GI24; B7-H5; Dies1; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha|
|Gene ID :||64115|
|mRNA Refseq :||NM_022153.2|
|Protein Refseq :||NP_071436.1|
|UniProt ID :||Q9H7M9|