|Product Overview:||Recombinant Human WASF1-VCA domain fused with GST tag was produced in E. coli.|
|Description:||The VCA-domains of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous) activate in vitro the nucleation ability of Arp 2/3, which is itself a constitutively inactive complex.
The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli, purified on GST-Sepharose further column purified by gel filtration.
|Form:||150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 250 µl ultrapure water to obtain a 1.0 mg/ml solution.|
|Purity:||>99% by scanning densitometry from Coomassie G-250 stained SDS-Gels.|
|Storage:||GST-VCA is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles.|
|Shipping:||At ambient temperature. Upon delivery store at -70 centigrade.|