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| Cat.No. : | WASF1-VCA domain-877H |
| Product Overview : | Recombinant Human WASF1-VCA domain fused with GST tag was produced in E. coli. |
| Description : | The VCA-domains of members of the Wiskott-Aldrich syndrome family protein, like WAVE (WASP family verprolin homologous) activate in vitro the nucleation ability of Arp 2/3, which is itself a constitutively inactive complex. The recombinant VCA-domain is supplied as GST-tagged peptide (~35 kDa) expressed in E. coli, purified on GST-Sepharose further column purified by gel filtration. |
| Source : | E. coli |
| Species : | Human |
| Tag : | GST |
| Form : | 150mM NaCl, 20mM Hepes pH 7.4, 1mM DTT and 5% sucrose, when reconstituted with 250 µl ultrapure water to obtain a 1.0 mg/ml solution. |
| Molecular Mass : | ~37.5kDa |
| Purity : | >99% by scanning densitometry from Coomassie G-250 stained SDS-Gels. |
| Storage : | GST-VCA is stored at –70 centigrade upon arrival will be stable in performance for at least 6 months from the date of purchase. The solubilized protein is kept on ice and should be stored at -20 centigrade in glycerol or CryoProtect (Cat.#: 6011-01). Avoid repeated freeze / thaw cycles. |
| Shipping : | At ambient temperature. Upon delivery store at -70 centigrade. |
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