Recombinant Human WNT3A

• Cat.No.: WNT3A-1700H

Specification

• Cat. No.: WNT3A-1700H
• Description: Wingless-type MMTV integration site family, member 3A, also known as WNT3A is an intercellular signaling molecule. WNT3A is expressed extensively in cells fated to give rise to embryonic mesoderm. Wnt-3a regulates survival, expansion, and maintenance of neural progenitors derived from embryonic stem cells. It has been reported that Wnt-3a supports self-renewal of embryonic stem cells and can maintain these cells in a pluripotent state. Wnt-3a is also considered a ligand that activates the beta-catenin-dependent pathway in Wnt signaling, which is implicated in numerous physiological events such as morphogenesis.
• Source: CHO.
• State Of Matter: Lyophilized.
• Purity: >75% as determined by SDS-PAGE under reducing conditions.
• Endotoxin: <0.1 EU/µg as determined by the LAL method.
• Molecular Weight: The predicted molecular weight of Recombinant Human WNT-3a (Ser19 - Lys352) is Mr 37.4 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 40 kDa.
• Formulation: The protein was 0.2 µm filtered and lyophilized from PBS, 1.0 mM EDTA, 0.5% CHAPS, pH 6.8.
• Biological Activity: The biological activity of Human WNT-3a was determined by its ability to promote alkaline phosphatase production by MC3T3E1 cells. The expected ED50 is 5-25 ng/ml.
• Storage And Stability: This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C for up to three months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
• Tag: Non
• Publication:
  Fibrinogen Induces RUNX2 Activity and Osteogenic Development from Human Pluripotent Stem Cells. (2016)
  Lineage-specific differentiation of osteogenic progenitors from pluripotent stem cells reveals the FGF1-RUNX2 association in neural crest-derived osteoprogenitors (2020)

Gene Information

• Gene Name: WNT3A wingless-type MMTV integration site family, member 3A [ Homo sapiens ]
• Synonyms: wingless-type MMTV integration site family, member 3A; MGC119418; MGC119419; MGC119420; WNT3A; OTTHUMP00000035713
• Gene ID: 89780
• mRNA Refseq: NM_033131
• Protein Refseq: NP_149122
• MIM: 606359
• UniProt ID: P56704
• Chromosome Location: 1q42
• Pathway: Basal cell carcinoma; Hedgehog signaling pathway; Melanogenesis; Pathways in cancer; Wnt signaling pathway
• Function: extracellular matrix structural constituent; protein binding; signal transducer activity

Reviews

07/15/2022

This protein is highly expressed and can efficiently synthesize a large number of target proteins in host cells.

06/03/2022

This protein has therapeutic effects on specific disease models and is a promising candidate for new drugs.

11/20/2019

The laboratory personnel feedback that the protein is easy to operate, can meet the experimental needs, and has a good cost performance.

Q&As

Does CDH16 protein interact with WNT3A protein?

No interaction between CDH16 protein and WNT3A protein has been found.

Which role of WNT3A protein in embryonic development?

The WNT3A protein plays an important role in embryonic development. It is involved in the formation of multiple embryonic organs, directed cell migration, and the establishment of tissue polarity.

Have regulators of WNT3A protein been identified?

Yes, multiple factors have been found in the WNT signaling pathway that regulate the expression and function of WNT3A protein, including Axin, APC, etc.

Where is the WNT3A protein in the Wnt signaling pathway?

The WNT3A protein is in the starting position in the Wnt signaling pathway, and it is able to bind to the Wnt receptor and initiate the signaling cascade.

What is the relationship between WNT3A protein and cancer stem cells?

WNT3A protein is closely related to cancer stem cells. It may be involved in the self-renewal and differentiation regulation of cancer stem cells, which is of great significance for tumor development and treatment.

How about the current clinical research progress on WNT3A protein?

Clinical research on WNT3A protein is still ongoing. Studies have shown the important role of WNT3A protein in tumors, neurological diseases, bone metabolism diseases, etc., and future research will further explore its application potential in disease diagnosis, treatment and prognosis assessment.