"WNT5A" Related Products

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Recombinant Human WNT5A protein

Cat.No.: WNT5A-19H
Product Overview: Recombinant Human WNT5A(Gln38-Lys380) was expressed in CHO.
Source: CHO
Species: Human
Predicted N Terminal: Asn44 & No results obtained: Gln38 predicted
Form: Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Bio-activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.1-0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
Molecular Mass: Predicted Molecular Mass: 38 kDa;SDS-PAGE: 45 kDa, reducing conditions.
Endotoxin: <1.0 EU per 1 μg of the protein by the LAL method.
Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Gene Name: WNT5A wingless-type MMTV integration site family, member 5A [ Homo sapiens ]
Official Symbol: WNT5A
Synonyms: WNT5A; wingless-type MMTV integration site family, member 5A; protein Wnt-5a; hWNT5A; WNT 5A protein; WNT-5A protein;
Gene ID: 7474
mRNA Refseq: NM_001256105
Protein Refseq: NP_001243034
MIM: 164975
UniProt ID: P41221
Chromosome Location: 3p21-p14
Pathway: Basal cell carcinoma, organism-specific biosystem; Basal cell carcinoma, conserved biosystem; Class B/2 (Secretin family receptors), organism-specific biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem;
Function: cytokine activity; frizzled binding; frizzled-2 binding; protein domain specific binding; receptor agonist activity; receptor binding; receptor tyrosine kinase-like orphan receptor binding; sequence-specific DNA binding transcription factor activity; transcription regulatory region DNA binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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