Recombinant Human WRN Protein, 517-1093, N-GST tagged

• Cat.No.: WRN-12H
• Product Overview: Recombinant human WRN (Werner syndrome ATP-dependent helicase), encompassing amino acids 517-1093. This construct contains an N-terminal GST-tag followed by a Thrombin cleavage site. The recombinant protein was affinity purified.

Specification

• Description: This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers.
• Source: Sf9 insect cells
• Species: Human
• Tag: N-GST
• Protein length: 517-1093
• Form: Aqueous buffer solution
• Molecular Mass: 92 kDa
• Purity: 0.9
• Notes: Avoid freeze/thaw cycles
• Usage: Thaw on ice and gently mix prior to use. DO NOT VORTEX. Perform a quick spin before opening. Aliquot into small volumes and flash freeze for long term storage. Avoid multiple freeze/thaw cycles.
• Storage: At least 6 months at -80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 200 mM NaCl, 2.2 mM KCl, 0.05% Tween-20, 10 mM glutathione, 1 mM TCEP, and 20% glycerol
• Shipping: At -80 centigrade

Gene Information

• Gene Name: WRN WRN RecQ like helicase [ Homo sapiens (human) ]
• Official Symbol: WRN
• Synonyms: WRN; WRN RecQ like helicase; RECQ3; RECQL2; RECQL3; bifunctional 3'-5' exonuclease/ATP-dependent helicase WRN; DNA helicase, RecQ-like type 3; Werner syndrome ATP-dependent helicase; Werner syndrome RecQ like helicase; Werner syndrome protein; Werner syndrome, RecQ helicase-like; exonuclease WRN; recQ protein-like 2; EC 3.6.4.12
• Gene ID: 7486
• mRNA Refseq: NM_000553
• Protein Refseq: NP_000544
• MIM: 604611
• UniProt ID: Q14191