Recombinant Human XPC Protein (496-734 aa), His-tagged
Cat.No. : | XPC-848H |
Product Overview : | Recombinant Human XPC Protein (496-734 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial. |
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Description : | Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Tris-based buffer, 50% glycerol |
Molecular Mass : | 31.5 kDa |
Protein length : | 496-734 aa |
AA Sequence : | SLPAASSSSSSSKRGKKMCSDGEKA EKRSIAGIDQWLEVFCEQEEKWVCV DCVHGVVGQPLTCYKYATKPMTYVV GIDSDGWVRDVTQRYDPVWMTVTRK CRVDAEWWAETLRPYQSPFMDREKK EDLEFQAKHMDQPLPTAIGLYKNHP LYALKRHLLKYEAIYPETAAILGYC RGEAVYSRDCVHTLHSRDTWLKKAR VVRLGEVPYKMVKGFSNRARKARLA EPQLREENDLGLFG |
Purity : | > 90% as determined by SDS-PAGE. |
Notes : | Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
Concentration : | A hardcopy of COA with concentration instruction is sent along with the products. |
Gene Name : | XPC xeroderma pigmentosum, complementation group C [ Homo sapiens ] |
Official Symbol : | XPC |
Synonyms : | XPC; RAD4; XPCC; p125; XP3; |
Gene ID : | 7508 |
mRNA Refseq : | NM_001145769 |
Protein Refseq : | NP_001139241 |
MIM : | 613208 |
UniProt ID : | Q01831 |
Products Types
◆ Recombinant Protein | ||
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Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionXPC is expressed ubiquitously but is especially important in skin cells exposed to UV radiation.
XPC plays a crucial role in nucleotide excision repair (NER), recognizing and initiating repair of bulky DNA lesions.
Genetic mutations in XPC are linked to Xeroderma Pigmentosum, a condition with extreme sensitivity to UV radiation and a high risk of skin cancers.
Dysfunction in XPC can lead to an increased risk of skin cancers and other malignancies.
By facilitating DNA repair, XPC helps maintain genomic stability and prevent mutations.
XPC is vital for repairing UV-induced DNA damage, preventing mutations and skin cancers.
Therapeutic strategies are focused on enhancing DNA repair mechanisms and preventing UV-induced skin damage.
Customer Reviews (3)
Write a reviewHigh-quality, perfect for experiments.
Consistent and reliable results.
Quick delivery, very satisfied.
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