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Recombinant Human XPC Protein (496-734 aa), His-tagged

Cat.No. : XPC-848H
Product Overview : Recombinant Human XPC Protein (496-734 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Partial.
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Description : Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.
Source : E. coli
Species : Human
Tag : His
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 31.5 kDa
Protein length : 496-734 aa
AA Sequence : SLPAASSSSSSSKRGKKMCSDGEKA EKRSIAGIDQWLEVFCEQEEKWVCV DCVHGVVGQPLTCYKYATKPMTYVV GIDSDGWVRDVTQRYDPVWMTVTRK CRVDAEWWAETLRPYQSPFMDREKK EDLEFQAKHMDQPLPTAIGLYKNHP LYALKRHLLKYEAIYPETAAILGYC RGEAVYSRDCVHTLHSRDTWLKKAR VVRLGEVPYKMVKGFSNRARKARLA EPQLREENDLGLFG
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Gene Name : XPC xeroderma pigmentosum, complementation group C [ Homo sapiens ]
Official Symbol : XPC
Synonyms : XPC; RAD4; XPCC; p125; XP3;
Gene ID : 7508
mRNA Refseq : NM_001145769
Protein Refseq : NP_001139241
MIM : 613208
UniProt ID : Q01831

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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In which cells or tissues is XPC expression most prominent? 05/09/2022

XPC is expressed ubiquitously but is especially important in skin cells exposed to UV radiation.

What is the primary role of XPC in DNA repair? 11/28/2020

XPC plays a crucial role in nucleotide excision repair (NER), recognizing and initiating repair of bulky DNA lesions.

Are there known genetic mutations in XPC linked to specific diseases? 08/15/2020

Genetic mutations in XPC are linked to Xeroderma Pigmentosum, a condition with extreme sensitivity to UV radiation and a high risk of skin cancers.

How is XPC dysfunction related to cancer development? 07/14/2020

Dysfunction in XPC can lead to an increased risk of skin cancers and other malignancies.

How does XPC contribute to maintaining genomic stability? 01/28/2020

By facilitating DNA repair, XPC helps maintain genomic stability and prevent mutations.

What is the relationship between XPC and UV radiation damage? 10/18/2019

XPC is vital for repairing UV-induced DNA damage, preventing mutations and skin cancers.

What therapeutic strategies are being explored involving XPC for disease prevention or treatment? 01/01/2019

Therapeutic strategies are focused on enhancing DNA repair mechanisms and preventing UV-induced skin damage.

Customer Reviews (3)

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Reviews
08/10/2022

    High-quality, perfect for experiments.

    06/18/2022

      Consistent and reliable results.

      03/16/2021

        Quick delivery, very satisfied.

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