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Recombinant Human YWHAZ 293 Cell Lysate

Cat.No. : YWHAZ-229HCL
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Description : Antigen standard for tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide (YWHAZ), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Tag : Non
Gene Name : YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide [ Homo sapiens ]
Official Symbol : YWHAZ
Synonyms : YWHAZ; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide , YWHAD; 14-3-3 protein zeta/delta; 14 3 3 delta; 14 3 3 zeta; KCIP 1; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; YWHAD; KCIP-1; 14-3-3-zeta; MGC111427; MGC126532; MGC138156;
Gene ID : 7534
mRNA Refseq : NM_001135699
Protein Refseq : NP_001129171
MIM : 601288
UniProt ID : P63104
Chromosome Location : 8q22.3
Pathway : Adaptive Immune System, organism-specific biosystem; Alpha6-Beta4 Integrin Signaling Pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Class I PI3K signaling events mediated by Akt, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem;

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Customer Reviews (3)

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Reviews
10/17/2022

    I greatly appreciate their commitment to ensuring my research is a success.

    01/01/2022

      The YWHAZ Protein offers a wide range of applications, making it highly versatile for various experimental settings.

      11/16/2020

        With the YWHAZ Protein, I have the confidence to generate critical insights and drive significant advancements in my field.

        Q&As (5)

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        How does YWHAZ contribute to cardiovascular diseases? 09/03/2021

        YWHAZ is involved in cardiovascular diseases by influencing processes like vascular smooth muscle cell proliferation and apoptosis.

        Are there diagnostic applications of YWHAZ in cancer detection? 04/11/2021

        Yes, elevated levels of YWHAZ protein have been detected in certain cancers, making it a potential biomarker for cancer diagnosis.

        Is YWHAZ a potential biomarker for cardiovascular diseases? 01/27/2018

        Preliminary studies suggest that YWHAZ levels may be altered in cardiovascular diseases, making it a candidate biomarker for further investigation.

        Can YWHAZ protein be targeted for cancer treatment? 03/27/2016

        Research suggests that targeting YWHAZ protein may be a viable strategy for developing anticancer therapies.

        Are there potential therapeutic interventions targeting YWHAZ in neurodegenerative disorders? 03/18/2016

        Investigational studies are exploring the modulation of YWHAZ as a potential therapeutic approach for neurodegenerative disorders.

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