Active Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, mFc-tagged, low endotoxin

• Cat.No.: CD28-336H
• Product Overview: Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein (Asn19-Pro152)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).In the region Asn19-Pro152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.

Specification

• Species: Human
• Source: HEK293
• Tag: mFc
• Protein Length: Asn 19 - Pro 152
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human / Cynomolgus / Rhesus macaque CD28, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag with a linear range of 0.1-5 μg/mL.
• Molecular Mass: The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
  >90% as determined by SEC-MALS.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD28 CD28 molecule [ Homo sapiens ]
• Official Symbol: CD28
• Synonyms: CD28; CD28 molecule; CD28 antigen (Tp44); T-cell-specific surface glycoprotein CD28; T cell specific surface glycoprotein; CD28 antigen; Tp44; MGC138290
• Gene ID: 940
• mRNA Refseq: NM_001243077
• Protein Refseq: NP_001230006
• MIM: 186760
• UniProt ID: P10747