Optional Service: Optional requirements on this protein
|Product Overview :
||Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein (Asn19-Pro152)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).In the region Asn19-Pro152, the AA sequence of Human, Cynomolgus and Rhesus macaque CD28 are homologus.
||T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
||Mouse IgG2a Fc
||Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
|Molecular Mass :
||The protein has a calculated MW of 42.0 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
|Protein length :
||Less than 0.1 EU per ug by the LAL method.
||>95% as determined by SDS-PAGE.
||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
|SDS-PAGE of CD28-336H :
Human/Cynomolgus/Rhesus macaque CD28, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of CD28-336H :
Immobilized Human/Cynomolgus/Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Human B7-2, Fc Tag with a linear range of 0.313-5 μg/mL (QC tested).
Immobilized Human/Cynomolgus/Rhesus macaque CD28, Mouse IgG2a Fc Tag, low endotoxin at 5 μg/mL (100 μL/well) can bind Human B7-1, Fc Tag with a linear range of 0.625-10 μg/mL (Routinely tested).