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Recombinant Mouse Abcg2 protein, His & T7-tagged

Cat.No. : Abcg2-5673M
Product Overview : Recombinant Mouse Abcg2 aa. (Leu63~Leu349 (Accession # Q7TMS5)) fused with N-terminal His & T7 tag was produced in E. coli cells.
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  • Gene Information
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Description : The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, the human protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. This protein likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure.
Source : E. coli
Species : Mouse
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 34.9kDa
Protein length : Leu63~Leu349 (Accession # Q7TMS5)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.8.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200μg/mL
Storage buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in sterile ddH2O.
Gene Name : Abcg2 ATP-binding cassette, sub-family G (WHITE), member 2 [ Mus musculus (house mouse) ]
Official Symbol : Abcg2
Synonyms : Abcg2; ATP-binding cassette, sub-family G (WHITE), member 2; MXR; ABCP; BCRP; MXR1; ABC15; Bcrp1; AI428558; ATP-binding cassette sub-family G member 2; breast cancer resistance protein 1 homolog; mitoxantrone resistance protein 1; urate exporter
Gene ID : 26357
mRNA Refseq : NM_011920.3
Protein Refseq : NP_036050.1
UniProt ID : Q7TMS5

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (20)

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What are the functional consequences of ABCG1 deficiency or overexpression? 01/17/2023

ABCG1 deficiency or overexpression can have functional consequences on cellular lipid metabolism. Deficiency of ABCG1 may lead to impaired cholesterol efflux and lipid accumulation, while overexpression of ABCG1 can enhance cholesterol efflux and improve lipid homeostasis.

Are there any known inhibitors or modulators of ABCG2 that could have therapeutic implications? 10/07/2022

Several inhibitors and modulators of ABCG2 have been identified, and they have potential therapeutic implications. These compounds can block the efflux function of ABCG2, thereby increasing the intracellular concentrations of co-administered drugs and overcoming ABCG2-mediated drug resistance. 

What is the fucntion of ABCG2 protein? 01/07/2021

It actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. In addition, it Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme

What is the clinical relevance of ABCG2 in cancer and its implications for chemotherapy? 09/10/2020

ABCG2 has clinical relevance in cancer due to its role in multidrug resistance. Overexpression of ABCG2 in cancer cells can lead to reduced intracellular drug concentrations, limiting the effectiveness of chemotherapy

Are there any genetic variations or polymorphisms in the ABCG2 gene associated with altered drug response or increased susceptibility to diseases? 08/03/2020

Yes, there are known genetic variations and polymorphisms in the ABCG2 gene associated with altered drug response and increased susceptibility to certain diseases. One well-studied polymorphism is the Q141K variant, which has been shown to affect ABCG2 function and alter drug pharmacokinetics. 

How is the expression of ABCG1 regulated in different cell types or tissues? 04/06/2020

The expression of ABCG1 can be regulated at the transcriptional level in response to changes in cellular cholesterol levels. Various transcription factors and signaling pathways, such as liver X receptors (LXRs) and peroxisome proliferator-activated receptors (PPARs), can influence ABCG1 expression.

Which species are the portein of ABCG2 present in? 10/11/2019

It presents in bovine, human, mouse, zebrafish and A.thaliana.

What are the specific substrates transported by ABCG2? 07/11/2019

ABCG2 transports a broad range of substrates, including chemotherapeutic drugs, such as mitoxantrone, topotecan, and methotrexate, as well as various endogenous molecules, such as heme, uric acid, and bilirubin. 

Are there any pharmacological approaches or strategies to enhance the efficacy of ABCG2 substrates in cancer treatment? 10/03/2018

One strategy involves using specific ABCG2 inhibitors to block its efflux function, thereby increasing the intracellular concentration of co-administered anticancer drugs. Another approach is to develop prodrugs that are selectively activated inside cancer cells, bypassing the efflux activity of ABCG2. Furthermore, combination therapies that target multiple pathways involved in drug resistance, including ABCG2, are being explored to overcome multidrug resistance in cancer.

Does ABCG2 play a role in the development and progression of neurodegenerative diseases? 08/27/2018

ABCG2 is expressed in the blood-brain barrier and plays a role in protecting the brain from potentially harmful substances. Dysregulation of ABCG2 expression or function has been implicated in the accumulation of neurotoxic compounds, such as amyloid-beta peptides, in neurodegenerative disorders like Alzheimer's disease.

How is ABCG2 expression regulated in different tissues and cell types? 04/17/2018

ABCG2 expression is controlled by various transcription factors and signaling pathways, including nuclear factor erythroid 2-related factor 2 (NRF2) and pregnane X receptor (PXR). Additionally, genetic and epigenetic factors can influence ABCG2 expression levels.

What is the mechanism of action of the ABCG1 protein? 12/06/2017

The ABCG1 protein functions as an ATP-binding cassette (ABC) transporter involved in cellular lipid metabolism. It promotes the efflux of cholesterol and other lipids from cells, contributing to cholesterol homeostasis.

How is ABCG2 involved in the efflux of anticancer drugs and the development of multidrug resistance? 11/30/2017

ABCG2 is involved in the efflux of anticancer drugs and contributes to the development of multidrug resistance. Cancer cells can overexpress ABCG2, which reduces the intracellular accumulation of chemotherapeutic agents and limits their effectiveness. This efflux activity of ABCG2 enables cancer cells to evade the cytotoxic effects of drugs, leading to treatment failure and disease recurrence.

Are there any tissue-specific differences in ABCG2 expression and function? 07/17/2017

Yes, there are tissue-specific differences in ABCG2 expression and function. ABCG2 is highly expressed in tissues with barrier functions, such as the intestines, liver, kidneys, and blood-brain barrier. This tissue-specific expression pattern reflects the role of ABCG2 in protecting these barriers from toxic compounds. 

Can ABCG2 polymorphisms influence drug response and toxicity in different populations? 05/24/2017

 Yes, ABCG2 polymorphisms can influence drug response and toxicity in different populations. Several genetic variations in the ABCG2 gene have been identified, some of which are associated with altered protein function and drug pharmacokinetics. These polymorphisms can affect the expression, transport activity, and substrate specificity of ABCG2, leading to variations in drug response and toxicity profiles. 

What is the impact of ABCG2 on the bioavailability and distribution of dietary phytochemicals or natural products? 03/05/2017

ABCG2 may impact the bioavailability and distribution of dietary phytochemicals or natural products. It is known to be involved in the efflux of certain dietary compounds, including flavonoids and polyphenols. ABCG2 activity can influence their absorption, metabolism, and tissue distribution, potentially affecting their bioactivity and health benefits

What is the role of ABCG2 in cellular transport and drug resistance? 01/15/2017

ABCG2 plays a crucial role in cellular transport and drug resistance. It is an efflux transporter that helps to eliminate various endogenous and exogenous compounds from cells, including drugs, toxins, and metabolites.

What is the role of ABCG2 in protecting against xenobiotic and environmental toxin exposure? 12/05/2016

ABCG2 plays a role in protecting against xenobiotic and environmental toxin exposure. It functions as a defense mechanism by actively pumping out harmful substances from cells, thereby reducing their intracellular concentrations. This protective role is particularly important in barrier tissues, such as the intestines and blood-brain barrier, where ABCG2 helps prevent the entry of toxins into the body or restrict their distribution to sensitive organs.

Does ABCG2 play a role in stem cell biology and tissue development? 09/06/2016

 ABCG2 has been found to play a role in stem cell biology and tissue development. It is expressed in various stem cell populations, including hematopoietic stem cells and neural stem cells. ABCG2 contributes to the maintenance and protection of stem cells by regulating the efflux of various substrates, including toxins and reactive oxygen species. Its activity helps to preserve the self-renewal capacity and differentiation potential of stem cells.

How does the substrated transported by ABCG2 affect drug disposition? 09/04/2016

The transport of these substrates by ABCG2 impacts drug disposition, tissue distribution, and elimination. ABCG2 is localized on the apical membrane of epithelial cells, where it contributes to the efflux of substrates into the luminal or extracellular space.

Customer Reviews (5)

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Reviews
02/14/2020

     its clear instructions provided a seamless user experience

    11/03/2017

      It remained active and retaining its functional properties even under varying storage conditions

      08/04/2017

        It played a crucial role in my experiments

        07/13/2017

          The protein product demonstrated remarkable specificity.

          03/12/2016

            The company's willingness to go the extra mile in resolving a shipping issue.

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