Active Recombinant Mouse Adipoq Protein, His-tagged

• Cat.No.: Adipoq-033M
• Product Overview: Purified recombinant protein of Mouse adiponectin, C1Q and collagen domain containing (Adipoq) with a N-His tag was expressed in Hi-5 insect.

Specification

• Description: Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
• Source: Insect Cell
• Species: Mouse
• Tag: His
• Bio-activity: Determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 4.0-6.0ug/mL.
• Molecular Mass: 35 kDa
• AA Sequence: RGHHHHHHHHVTTTEELAPALVPPP KGTCAGWMAGIPGHPGHNGTPGRDG RDGTPGEKGEKGDAGLLGPKGETGD VGMTGAEGPRGFPGTPGRKGEPGEA AYVYRSAFSVGLETRVTVPNVPIRF TKIFYNQQNHYDGSTGKFYCNIPGL YYFSYHITVYMKDVKVSLFKKDKAV LFTYDQYQEKNVDQASGSVLLHLEV GDQVWLQVYGDGDHNGLYADNVNDS TFTGFLLYHDTN
• Endotoxin: Endotoxin level is < 0.1 ng/µg of protein (< 1 EU/µg)
• Purity: >95%, as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for at least 6 months from date of receipt under proper storage and handling conditions.
• Storage: Store at -80 centigrade.
• Storage Buffer: Lyophilized from a 0.2 µM filtered solution of 20mM phosphate buffer,100mM NaCl, pH 7.2
• Reconstitution: Resuspend the protein in the desired concentration in proper buffer.

Gene Information

• Gene Name: Adipoq adiponectin, C1Q and collagen domain containing [ Mus musculus (house mouse) ]
• Official Symbol: Adipoq
• Synonyms: Adipoq; adiponectin, C1Q and collagen domain containing; Ad; APN; Acdc; Adid; apM1; 30kDa; GBP28; adipo; Acrp30; adiponectin; 30 kDa adipocyte complement-related protein; adipocyte complement related protein; adipocyte complement-related 30 kDa protein; adipocyte, C1Q and collagen domain containing; adipocyte, C1q and collagen domain-containing protein; adipocyte-specific protein AdipoQ; adiponectin d
• Gene ID: 11450
• mRNA Refseq: NM_009605
• Protein Refseq: NP_033735
• UniProt ID: Q60994