Recombinant Mouse Afg1l Protein, Myc/DDK-tagged

• Cat.No.: Afg1l-1548M
• Product Overview: Purified recombinant protein of mouse full-length AFG1 like ATPase (Afg1l), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Putative mitochondrial ATPase. Plays a role in mitochondrial morphology and mitochondrial protein metabolism. Promotes degradation of excess nuclear-encoded complex IV subunits (COX4I1, COX5A and COX6A1) and normal activity of complexes III and IV of the respiratory chain. Mediates mitochondrial translocation of TP53 and its transcription-independent apoptosis in response to genotoxic stress.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 54.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Afg1l AFG1 like ATPase [ Mus musculus (house mouse) ]
• Official Symbol: Afg1l
• Synonyms: Afg1l; AFG1 like ATPase; Lac; Lace1; CG8520; AFG1-like ATPase; lactation elevated 1; lactation elevated protein 1
• Gene ID: 215951
• mRNA Refseq: NM_145743
• Protein Refseq: NP_665686
• UniProt ID: Q3V384

Reviews

05/22/2022

Elucidating protein-protein interactions in autophagy flux for cellular homeostasis.

03/25/2019

Deciphering protein-protein interactions in mitochondrial dysfunction for metabolic disorders.

12/03/2018

Probing protein-protein interactions in neuronal development for synaptic connectivity.

Q&As

Are there any genetic variations or mutations in the gene encoding AFG1L protein that impact its expression or function, and what are the implications for mitochondrial dysfunction or disease susceptibility?

Genetic variations or mutations in the gene encoding AFG1L protein may impact its expression or function, influencing mitochondrial dysfunction or disease susceptibility.

How is the expression of AFG1L protein regulated, and what factors or signaling pathways control its expression?

The expression of AFG1L protein is regulated by specific factors and signaling pathways.

How does AFG1L protein contribute to cellular processes such as energy metabolism, oxidative stress response, or mitochondrial dynamics, and what are the underlying molecular mechanisms?

AFG1L protein contributes to cellular processes such as energy metabolism, oxidative stress response, or mitochondrial dynamics, and the underlying molecular mechanisms are currently being investigated.

What experimental techniques or assays have been used to study the functional significance of AFG1L protein, such as mitochondrial respiration assays, electron microscopy, or gene knockout models?

Various experimental techniques or assays, such as mitochondrial respiration assays, electron microscopy, or gene knockout models, have been used to study the functional significance of AFG1L protein.

Does AFG1L protein interact with specific molecules or participate in protein complexes, and what are the functional implications of these interactions?

AFG1L protein may interact with specific molecules or participate in protein complexes, which have functional implications in cellular processes.

Are there any known post-translational modifications or regulatory mechanisms that modulate the activity or stability of AFG1L protein?

Post-translational modifications and regulatory mechanisms may modulate the activity or stability of AFG1L protein.

What is the subcellular localization of AFG1L protein, and how can it be experimentally determined?

The subcellular localization of AFG1L protein can be experimentally determined using techniques such as immunofluorescence microscopy or subcellular fractionation.

Can AFG1L protein be targeted or manipulated to modulate mitochondrial function or treat mitochondrial disorders, and what are the potential implications for therapeutic interventions?

AFG1L protein holds potential as a target for modulating mitochondrial function or treating mitochondrial disorders, and further research is needed to explore its implications for therapeutic interventions.

What is the role of AFG1L protein in mitochondrial function or quality control, and how can its function be investigated experimentally?

AFG1L protein plays a role in mitochondrial function or quality control, and its function can be investigated using techniques like mitochondrial respiration assays or electron microscopy.

How does dysregulation or dysfunction of AFG1L protein affect mitochondrial homeostasis, cellular energetics, or disease development?

Dysregulation or dysfunction of AFG1L protein can disrupt mitochondrial homeostasis, impair cellular energetics, and contribute to disease development.