Recombinant Mouse Akt2 Protein, MYC/DDK-tagged

• Cat.No.: Akt2-567M
• Product Overview: Purified recombinant protein of full-length mouse thymoma viral proto-oncogene 2 (Akt2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: One of the few specific substrates of AKT2 identified so far is PITX2. Phosphorylation of PITX2 impairs its association with the CCND1 mRNA-stabilizing complex thus shortening the half-life of CCND1. AKT2 seems also to be the principal isoform responsible of the regulation of glucose uptake. Phosphorylates C2CD5 on 'Ser-197' during insulin-stimulated adipocytes. AKT2 is also specifically involved in skeletal muscle differentiation, one of its substrates in this process being ANKRD2. Phosphorylates CLK2 on 'Thr-343'.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 56.2 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Akt2 thymoma viral proto-oncogene 2 [ Mus musculus (house mouse) ]
• Official Symbol: Akt2
• Synonyms: Akt2; thymoma viral proto-oncogene 2; PKB; PKBbeta; AW554154; 2410016A19Rik; RAC-beta serine/threonine-protein kinase; PKB beta; RAC-PK-beta; protein kinase Akt-2; protein kinase B, beta; EC 2.7.11.1
• Gene ID: 11652
• mRNA Refseq: NM_007434
• Protein Refseq: NP_031460
• UniProt ID: Q60823