Recombinant Mouse Alkbh8 Protein, Myc/DDK-tagged

• Cat.No.: Alkbh8-1603M
• Product Overview: Purified recombinant protein of mouse full-length alkB homolog 8, tRNA methyltransferase (Alkbh8), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
• Molecular Mass: 74.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Alkbh8 alkB homolog 8, tRNA methyltransferase [ Mus musculus (house mouse) ]
• Official Symbol: Alkbh8
• Synonyms: ALKBH8; alkB, alkylation repair homolog 8 (E. coli); alkylated DNA repair protein alkB homolog 8; probable alpha-ketoglutarate-dependent dioxygenase ABH8; tRNA (carboxymethyluridine(34)-5-O)-methyltransferase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; Abh8; 4930562C03Rik; 8030431D03Rik; 9430088N01Rik; MGC10235
• Gene ID: 67667
• mRNA Refseq: NM_026303
• Protein Refseq: NP_080579
• UniProt ID: Q80Y20