Recombinant Mouse Angiopoietin-Like 7, His-tagged Angptl7-555M

Recombinant Mouse Angiopoietin-Like 7, His-tagged


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Cat.No. : Angptl7-555M Optional Service: Optional requirements on this protein
Product Overview : Recombinant Mouse Angptl7protein was expressed in Chinese Hamster Ovary cell line with a C-terminal 10-Histag. Met1-Pro337.
Description : Angptl7, also knownas CornealDerived Transcript 6 (CDT6), is a secreted, 45-50 kDa glycoproteinmember of the angiopoietinlike family of molecules. Members of this proteinfamily contain an N-terminal coiled coil domain and a Cterminal fibrinogenlike domain. Mature mouse Angptl7 is 316 amino acids (aa) in length. Itshares 88% and 98% aa sequence identity with human and rat Angptl7,respectively. The Angptl7 monomer forms homotetramers via its coiled coildomain. Angptl7 is expressed in the corneal stroma, trabecular meshwork, and sclera.Its production is upregulated in trabecular meshwork cells by glucocorticoidsand TGFβ and in cartilage by TNFα.
Source : Chinese HamsterOvary cell line, CHO-derived.
Form : Lyophilized from a0.2 μm filtered solution in NaH2PO4 and NaCl with BSA as a carrier protein.
N-terminalSequence : Gln22 predicted
Molecular Weight : 37.9 kDa
Activity : Measured by itsability to promote the expansion of E16 rat liver mononuclear cells in vitro,in the presence of Recombinant Mouse SCF, Recombinant Mouse Thrombopoietin, andRecombinant Mouse Flt3 Ligand. The ED50 for this effect istypically 60-240 ng/mL in the presence of a crosslinking MouseAntipolyHistidine Monoclonal Antibody.
Endotoxin Level : < 1.0EU per 1 μg of the protein by the LAL method.
SDS-PAGE : 45-55kDa, reducing conditions.
Purity : > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution : 500μg/mL in PBS.
Storage : Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.
Gene Name : Angptl7 angiopoietin-like 7 [ Musmusculus ]
Official Symbol : Angptl7
Synonyms : Angptl7;angiopoietin-like 7; angiopoietin-related protein 7;angiopoietin-like protein 7
Gene ID : 654812
mRNA Refseq : NM_001039554
Protein Refseq : NP_001034643
UniProt ID : Q8R1Q3
Chromosome Location : 4 E1; 4
Function : receptor binding
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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