Recombinant Mouse Ankfy1 Protein, MYC/DDK-tagged

• Cat.No.: Ankfy1-603M
• Product Overview: Purified recombinant protein of full-length mouse ankyrin repeat and FYVE domain containing 1 (Ankfy1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Ubiquitous expression in CNS E18 (RPKM 14.1), large intestine adult (RPKM 13.9) and 28 other tissues.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 128.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ankfy1 ankyrin repeat and FYVE domain containing 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ankfy1
• Synonyms: Ankfy1; ankyrin repeat and FYVE domain containing 1; Ankhzn; ZFYVE14; mKIAA1255; rabankyrin-5; Rabankyrin-5; ankyrin repeat and FYVE domain-containing protein 1; ankyrin repeat hooked to a zinc finger motif; ankyrin repeat hooked to zinc finger motif; ankyrin repeats hooked to a zinc finger motif; rank-5
• Gene ID: 11736
• mRNA Refseq: NM_009671
• Protein Refseq: NP_033801
• UniProt ID: Q810B6

Reviews

05/25/2021

its high quality and purity ensure accurate and reliable results.

06/06/2020

The ANKFY1 protein exhibits several valuable attributes that make it an excellent choice for my trials.

05/11/2016

the stability of the ANKFY1 protein enables me to conduct experiments over an extended period, reducing the need for frequent restocking and promoting experimental consistency.

04/23/2016

With the manufacturer's unwavering dedication to delivering quality products and unparalleled technical support, I am confident in their ability to help me overcome any challenges and propel my research forward.

Q&As

What are the functions of ANKFY1 protein?

The functions of ANKFY1 protein are not well-characterized. It contains ankyrin repeat and FYVE domain, suggesting potential involvement in protein-protein interactions and membrane trafficking processes. However, more experimental studies are required to establish its exact roles and molecular functions.

Is ANKFY1 protein conserved across different species?

ANKFY1 protein is evolutionarily conserved across various species, including mammals, amphibians, birds, and fish. This conservation suggests that ANKFY1 may play important roles in cellular processes that are conserved throughout evolution.

Are there any known disease associations or clinical implications of ANKFY1 protein?

Currently, there are no known disease associations or clinical implications specifically linked to ANKFY1 protein. Further studies are needed to investigate potential disease-related roles or clinical significance of ANKFY1 in various pathological conditions.

Are there any known partners or binding proteins for ANKFY1 protein?

As of now, there are no known specific binding partners or interacting proteins for ANKFY1 protein. Further studies are needed to identify and characterize its binding partners and determine the molecular interactions it participates in.

Is ANKFY1 protein involved in any signaling pathways?

ANKFY1 protein has been implicated in the regulation of PI3K/Akt signaling pathway, which is important for cell growth, survival, and metabolism. ANKFY1's FYVE domain allows it to bind to PI3P, suggesting a potential role in modulating this pathway.

How is ANKFY1 protein localized within cells?

The subcellular localization of ANKFY1 protein has not been extensively characterized. However, bioinformatics analysis suggests it may localize to the cytoplasm and cell membranes due to the presence of the FYVE domain, which typically binds to phosphoinositides in membranes. Experimental studies are needed to confirm its precise localization.

Are there any animal models available to study ANKFY1 protein function?

Currently, there are no specific animal models available to study the function of ANKFY1 protein. However, researchers may use knockout or knockdown approaches in mammalian models to investigate the effects of ANKFY1 loss-of-function.

Are there any resources available for further research on ANKFY1 protein?

Yes, various resources can be utilized for further research on ANKFY1 protein. These include databases such as UniProt, NCBI Gene, and PubMed, which provide information about protein structure, function, and publications related to ANKFY1. Additionally, conducting literature searches and exploring scientific journals may provide more information on ANKFY1 and its current research progress.

Is ANKFY1 protein associated with any diseases or disorders?

The ANKFY1 protein, also known as Ankyrin Repeat and FYVE Domain Containing 1, is not extensively studied in the context of diseases or disorders. Limited information is available regarding its associations with specific pathological conditions. Further research is needed to determine its potential roles in diseases.

Are there any known interacting partners of ANKFY1 protein?

Yes, ANKFY1 protein has been reported to interact with various proteins. Some of the known interacting partners include RAB5, a small GTPase involved in endocytosis and early endosome trafficking, and MICAL-L1, a protein associated with microtubules and membrane trafficking.

Does ANKFY1 protein have any known functional domains?

ANKFY1 protein contains several functional domains, including ankyrin repeats and a FYVE domain. Ankyrin repeats are involved in protein-protein interactions, while the FYVE domain is associated with binding to phosphatidylinositol 3-phosphate (PI3P)-containing membranes.

Are there any orthologs of ANKFY1 protein in other species?

Yes, ANKFY1 protein has orthologs in various species including mammals, birds, reptiles, amphibians, and several invertebrates. However, the homologs may have variations in sequence and function.

Are there any known genetic variations or mutations in the ANKFY1 gene?

Currently, there is no specific information available regarding genetic variations or mutations in the ANKFY1 gene. Comprehensive studies focusing on genetic variations and their potential implications need to be conducted to provide more insights into this aspect.

Is ANKFY1 protein expressed in specific tissues or organs?

Information regarding the tissue-specific expression of ANKFY1 protein is limited. It is believed to be expressed in multiple tissues, including the brain, based on mRNA expression data from public databases. However, more detailed studies are needed to precisely determine its tissue-specific expression patterns.

What are the functions of ANKFY1 protein in cellular processes?

The exact functions of ANKFY1 protein are still being investigated. However, it is suggested to play roles in endocytic trafficking, vesicle formation, and membrane trafficking. ANKFY1 may also be involved in autophagy, a cellular process responsible for recycling cellular components.

Can ANKFY1 protein interact with other molecules?

The specific molecular interactions of ANKFY1 protein have not been extensively investigated. It may potentially interact with proteins through its ankyrin repeat domains, which are known to mediate protein-protein interactions. However, further research is required to identify its binding partners and elucidate its functional interactions.

Can ANKFY1 protein be targeted for therapeutic purposes?

The therapeutic targeting of ANKFY1 protein has not been extensively explored. However, its potential involvement in cellular processes and protein interactions suggests that it could be a candidate for future therapeutic targeting. Further research is needed to elucidate its specific roles and potential therapeutic applications.

Is ANKFY1 protein involved in any cellular pathways or processes?

The cellular pathways or processes in which ANKFY1 protein is involved are not well-documented. The presence of ankyrin repeat and FYVE domain suggests potential involvement in membrane-associated processes and protein-protein interactions. However, further research is required to determine its specific roles in cellular pathways.