Recombinant Mouse Arf1 Protein, MYC/DDK-tagged Arf1-634M

Recombinant Mouse Arf1 Protein, MYC/DDK-tagged

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Recombinant Mouse Arf1 Protein, MYC/DDK-tagged

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Cat.No. : Arf1-634M
Product Overview : Purified recombinant protein of full-length mouse ADP-ribosylation factor 1 (Arf1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
Description : GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles. The GTP-bound form interacts with PICK1 to limit PICK1-mediated inhibition of Arp2/3 complex activity; the function is linked to AMPA receptor (AMPAR) trafficking, regulation of synaptic plasicity of excitatory synapses and spine shrinkage during long-term depression (LTD).
Source : HEK293T
Species : Mouse
Tag : MYC/DDK
Molecular Mass : 20.7 kDa
Purity : >80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Arf1 ADP-ribosylation factor 1 [ Mus musculus (house mouse) ]
Official Symbol : Arf1
Synonyms : Arf1; ADP-ribosylation factor 1; ADP-ribosylation factor 1
Gene ID : 11840
mRNA Refseq : NM_001130408
Protein Refseq : NP_001123880
UniProt ID : P84078

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