Recombinant Mouse Arih2 Protein, Myc/DDK-tagged

• Cat.No.: Arih2-1700M
• Product Overview: Purified recombinant protein of mouse full-length ariadne RBR E3 ubiquitin protein ligase 2 (Arih2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3. Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets. The initial ubiquitin is then elongated. E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex. Mediates 'Lys-6', 'Lys-48'- and 'Lys-63'-linked polyubiquitination. May play a role in myelopoiesis.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 57.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Arih2 ariadne RBR E3 ubiquitin protein ligase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Arih2
• Synonyms: ARIH2; ariadne homolog 2 (Drosophila); E3 ubiquitin-protein ligase ARIH2; ARI-2; ariadne 2; protein ariadne-2 homolog; ubcM4-interacting protein 48; ARI2; UIP48; TRIAD1; AI843547
• Gene ID: 23807
• mRNA Refseq: NM_011790
• Protein Refseq: NP_035920
• UniProt ID: Q9Z1K6