Recombinant Mouse Arpin Protein, Myc/DDK-tagged

• Cat.No.: Arpin-1728M
• Product Overview: Purified recombinant protein of mouse full-length actin-related protein 2/3 complex inhibitor (Arpin), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competetive with nucleation promoting factors. Participates in an incoherent feedforward loop at the lamellipodium tip where it inhibits the ARP2/2 complex in response to Rac signaling and where Rac also stimulates actin polymerization through the WAVE complex. Involved in steering cell migration by controlling its directional persistence.
• Molecular Mass: 25.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Arpin actin-related protein 2/3 complex inhibitor [ Mus musculus (house mouse) ]
• Official Symbol: Arpin
• Synonyms: Arpin; actin-related protein 2/3 complex inhibitor; 2610034B18Rik; arpin; UPF0552 protein C15orf38 homolog; arp2/3 inhibition protein
• Gene ID: 70420
• mRNA Refseq: NM_027420
• Protein Refseq: NP_081696
• UniProt ID: Q9D0A3