Recombinant Mouse Ascl1 Protein, Myc/DDK-tagged

• Cat.No.: Ascl1-1739M
• Product Overview: Purified recombinant protein of mouse full-length achaete-scute family bHLH transcription factor 1 (Ascl1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 25.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ascl1 achaete-scute family bHLH transcription factor 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ascl1
• Synonyms: ASCL1; achaete-scute complex homolog 1 (Drosophila); achaete-scute homolog 1; ASH-1; mASH-1; mammalian achaete scute homolog 1; achaete-scute complex homolog-like 1; ASH1; Mash1; bHLHa46; AI225900
• Gene ID: 17172
• mRNA Refseq: NM_008553
• Protein Refseq: NP_032579
• UniProt ID: Q02067