Recombinant Mouse AXL protein, His-tagged

• Cat.No.: Axl-626M
• Product Overview: Recombinant Mouse AXL protein(NP_033491.2)(Met1-Pro443), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant mouse AXL consists of 435 amino acids and has a predicted molecular mass of 47.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmAXL is approximately 60-65 kDa due to glycosylation.
• Protein length: Met1-Pro443
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Axl AXL receptor tyrosine kinase [ Mus musculus ]
• Official Symbol: AXL
• Synonyms: AXL; AXL receptor tyrosine kinase; tyrosine-protein kinase receptor UFO; ufo oncogene homolog; adhesion-related kinase; Ark; Ufo; Tyro7; AI323647
• Gene ID: 26362
• mRNA Refseq: NM_001190974
• Protein Refseq: NP_001177903

Citation

Harnessing MerTK agonism for targeted therapeutics

Journal: mAbs    PubMed ID: 31852344    Data: 2022/12/14

Authors: Vivekananda Kedage, Diego Ellerman, Minhong Yan

Article Snippet:ELISA was carried out to determine the specificity of MerTK bispecific antibodies for other members of TAM family.ELISA was carried out to determine the specificity of MerTK bispecific antibodies for other members of TAM family.. Briefly, 1ug/ml of human MerTK (Genentech), human AXL (Creative BioMart, Catalog AXL-769H), human TYRO3 (Creative BioMart, Catalog TYRO3-195H), mouse MerTK (R&D systems, Catalog 591-MR-100), mouse AXL (Creative BioMart, Catalog AXL-626M), mouse TYRO3 (R&D systems, Catalog 759-DT-100) were coated onto ELISA plates.. The plates were blocked with SuperBlock (TBS) blocking buffer (Thermo Fisher, Catalog 37535). hIgG1 and mIgG2a versions of bispecific antibodies were added followed by horseradish peroxidase (HRP)-conjugated anti-human IgG (Jackson laboratories, Catalog 709-035-149) and HRP-conjugated anti-mouse IgG (Jackson laboratories, Catalog 715-035-151) at 1:20,000 dilution.The plates were blocked with SuperBlock (TBS) blocking buffer (Thermo Fisher, Catalog 37535). hIgG1 and mIgG2a versions of bispecific antibodies were added followed by horseradish peroxidase (HRP)-conjugated anti-human IgG (Jackson laboratories, Catalog 709-035-149) and HRP-conjugated anti-mouse IgG (Jackson laboratories, Catalog 715-035-151) at 1:20,000 dilution.

Publication :

Harnessing MerTK agonism for targeted therapeutics (2020)