Recombinant Mouse B3gat3 Protein, Myc/DDK-tagged

• Cat.No.: B3gat3-1801M
• Product Overview: Purified recombinant protein of mouse full-length beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (B3gat3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 37.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: B3gat3 beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) [ Mus musculus (house mouse) ]
• Official Symbol: B3gat3
• Synonyms: B3GAT3; beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I); galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3; glcAT-I; glcUAT-I; glucuronyltransferase I; glucuronosyltransferase I; glucuronosyltransferase-I; UDP-GlcUA:Gal beta-1,3-Gal-R glucuronyltransferase; 2810405M13Rik
• Gene ID: 72727
• mRNA Refseq: NM_024256
• Protein Refseq: NP_077218
• UniProt ID: P58158