Recombinant Mouse B4galt1 overexpression lysate

• Cat.No.: B4galt1-07HCL
• Product Overview: Recombinant mouse B4galt1 HEK293T cell transient overexpression lysate (as WB positive control) with a C-Myc/DDK tag was expressed in HEK293T.
  Preparation: HEK293T cells in 10-cm dishes were transiently transfected. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. To facilitate transportation and protein, the products are supplied as lyophilized proteins.
  Components: 1 vial of 20 μg lyophilized gene specific transient over-expression cell lysate

Specification

• Description: This gene encodes two distinct enzyme isoforms, a long membrane-bound form and a short soluble form. These alternate isoforms are thought to be produced through alternative nested transcription initiation and different in-frame start codon usage. These enzymes catalyze the transfer of galactose to acceptor sugars, such as N-acetylglucosamine and glucose. The long form of this enzyme is localized to the trans-Golgi membrane and is involved in glycoconjugate biosynthesis. The short form functions in lactose biosynthesis though formation of a heterodimer with alpha-lactalbumin.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 44.4 kDa
• Stability: Lysate samples are stable for 12 months from date of receipt when stored at -20 centigrade. Avoid repeated freeze-thaw cycles after reconstitution.
• Storage: Upon receiving, store the sample at -20 centigrade.
• Reconstitution: Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Shipping: The lysate can be shipped at ambient temperature.

Gene Information

• Gene Name: B4galt1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: B4galt1
• Synonyms: B4galt1; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1; Ga; GalT; Ggtb; Ggtb2; b4Gal-T1; B-1,4-GalT; B-1,4-GalT1; beta4Gal-T1; beta-1,4-GalT; beta-1,4-GalT1; beta-1,4-galactosyltransferase 1; N-acetyllactosamine synthase; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; b1,4-Galactosyltransferase I; beta-1,4-GalTase 1; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; galactosyltransferase 2 beta 1, 4; glycoprotein galactosyltransferase beta 1, 4; lactose synthase A protein; nal synthase; neolactotriaosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.22; EC 2.4.1.275; EC 2.4.1.38; EC 2.4.1.90
• Gene ID: 14595
• mRNA Refseq: NM_022305
• Protein Refseq: NP_071641
• UniProt ID: P15535

Reviews

10/14/2021

Quick shipping, excellent quality.

10/03/2021

High quality, excellent performance.

05/16/2021

Reliable, good for research.

Q&As

How does B4GALT1 contribute to the synthesis of glycoproteins and glycolipids?

It synthesizes lactosylceramide, a building block for complex glycosphingolipids in glycoprotein and glycolipid synthesis.

What is the primary function of B4GALT1 in cellular processes?

B4GALT1 is crucial for glycosylation, attaching galactose to proteins and lipids.

In which cells or tissues is B4GALT1 predominantly expressed?

B4GALT1 is widely expressed but has higher activity in the liver, kidney, and intestine.

What therapeutic strategies are being explored targeting B4GALT1 for disease treatment?

Therapeutic strategies include targeting B4GALT1 to modulate its activity in cancer and other diseases.

How is B4GALT1 implicated in cancer progression and metastasis?

Altered B4GALT1 expression is linked to cancer, influencing tumor growth and metastasis.

Are there known genetic mutations in B4GALT1 associated with specific diseases or conditions?

Genetic mutations in B4GALT1 are associated with some rare metabolic disorders.

What role does B4GALT1 play in immune system function?

It modulates immune responses by altering glycosylation patterns on immune cells.