Recombinant Mouse BLM Protein (684-859 aa), His-Myc-tagged
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| Cat.No. : | BLM-2206M |
| Product Overview : | Recombinant Mouse BLM Protein (684-859 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Neuroscience. Protein Description: Partial. |
| Description : | Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. Negatively regulates sister chromatid exchange (SCE). |
| Source : | E. coli |
| Species : | Mouse |
| Tag : | His-Myc |
| Form : | Tris-based buffer,50% glycerol |
| Molecular Mass : | 24.7 kDa |
| Protein length : | 684-859 aa |
| AA Sequence : | INAALLGEDC FILMPTGGGK SLCYQLPACV SPGVTIVISP LRSLIVDQVQ KLTSFDIPAT YLTGDKTDSE AANIYLQLSK KDPIIKLLYV TPEKVCASNR LISTLENLYE RKLLARFVID EAHCVSQWGH DFRQDYKRMN MLRQKFPSVP VMALTATANP RVQKDILTQL KILRPQ |
| Purity : | > 90% as determined by SDS-PAGE. |
| Notes : | Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : | A hardcopy of COA will be sent along with the products. Please refer to it for detailed information. |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. |
| Gene Name : | Blm Bloom syndrome, RecQ helicase-like [ Mus musculus ] |
| Official Symbol : | BLM |
| Synonyms : | BLM; Bloom syndrome protein homolog; mBLM; recQ helicase homolog; Bloom syndrome homolog; |
| Gene ID : | 12144 |
| mRNA Refseq : | NM_001042527 |
| Protein Refseq : | NP_001035992 |
| UniProt ID : | O88700 |
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