Recombinant Mouse BLMH protein(Asn2-Glu455), His-tagged

• Cat.No.: Blmh-1190M
• Product Overview: Recombinant Mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed in E. coli, with a polyhistide tag at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Protein length: Asn2-Glu455
• Form: Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.
• Molecular Mass: The recombinant mouse BLMH consisting of 461 amino acids and has a calculated molecular mass of 53.3 kDa. rmBLMH migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Blmh bleomycin hydrolase [ Mus musculus ]
• Official Symbol: BLMH
• Synonyms: BLMH; bleomycin hydrolase; BLM hydrolase; Bh; Bmh; AI035728; MGC36899; MGC37104; MGC39026
• Gene ID: 104184
• mRNA Refseq: NM_178645
• Protein Refseq: NP_848760