Recombinant Mouse Brf2 Protein, Myc/DDK-tagged

• Cat.No.: Brf2-1893M
• Product Overview: Purified recombinant protein of mouse full-length BRF2, RNA polymerase III transcription initiation factor 50kDa subunit (Brf2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Contributes to the regulation of gene expression; functions as activator in the absence of oxidative stress. Down-regulates expression of target genes in response to oxidative stress. Overexpression protects cells against apoptosis in response to oxidative stress.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 47.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Brf2 BRF2, RNA polymerase III transcription initiation factor 50kDa subunit [ Mus musculus (house mouse) ]
• Official Symbol: Brf2
• Synonyms: BRF2; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like; transcription factor IIIB 50 kDa subunit; BRF-2; B-related factor 2; BRFU; AU018504; AU022602; TFIIIB50; 2700059M06Rik; 5730512K07Rik
• Gene ID: 66653
• mRNA Refseq: NM_025686
• Protein Refseq: NP_079962
• UniProt ID: Q3UAW9