Recombinant Mouse BTLA protein(Met1-Gly176), hFc-tagged

• Cat.No.: BTLA-8698M
• Product Overview: Recombinant Mouse BTLA (AAI08964.1, with mutations K173E) (Met1-Gly176) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc
• Protein length: Met1-Gly176
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized mouse BTLA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated mouse HVEM-Fch, The EC50 of biotinylated mouse HVEM-Fch is 15.7-36.7 ng/ml.
• Molecular Mass: The recombinant mouse BTLA /Fc is a disulfide-linked homodimer. The reduced monomer comprises 388 amino acids and has a predicted molecular mass of 437 kDa. The apparent molecular mass of the protein is approximately 57-62 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Btla B and T lymphocyte associated [ Mus musculus ]
• Official Symbol: BTLA
• Synonyms: BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; A630002H24; MGC124217; MGC124218
• Gene ID: 208154
• mRNA Refseq: NM_001037719
• Protein Refseq: NP_001032808

Reviews

01/25/2023

Very satisfied with a buying experience! Worth it!

04/01/2022

The staff actively communicated with me and solved many technical problems.

03/28/2022

The BTLA proteins purchased from your company is suitable for ELISA.

Q&As

What are the effects of inhibiting BTLA expression?

Inhibition of BTLA expression leads to disruption of autoimmune homeostasis in the organism.

Where is BTLA located?

BTLA is localized in the cell membrane.

What organs are BTLA expressed in?

BTLA is widely expressed in lymphocytes of the bone marrow, thymus and spleen.

What is the biological function of BTLA?

BTLA regulates T and B lymphocyte expression and plays a negative regulatory role in the activation phase of T lymphocytes.