Recombinant Mouse BTLA protein(Met1-Gly176), hFc-tagged

Cat.No. : BTLA-8698M
Product Overview : Recombinant Mouse BTLA (AAI08964.1, with mutations K173E) (Met1-Gly176) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.
Availability July 25, 2024
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Source : HEK293
Species : Mouse
Tag : Fc
Protein length : Met1-Gly176
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : Immobilized mouse BTLA-Fc at 10 μg/ml (100 μl/well) can bind biotinylated mouse HVEM-Fch, The EC50 of biotinylated mouse HVEM-Fch is 15.7-36.7 ng/ml.
Molecular Mass : The recombinant mouse BTLA /Fc is a disulfide-linked homodimer. The reduced monomer comprises 388 amino acids and has a predicted molecular mass of 437 kDa. The apparent molecular mass of the protein is approximately 57-62 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 90 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
Gene Name : Btla B and T lymphocyte associated [ Mus musculus ]
Official Symbol : BTLA
Synonyms : BTLA; B and T lymphocyte associated; B- and T-lymphocyte attenuator; B and T lymphocyte attenuator; B- and T-lymphocyte-associated protein; A630002H24; MGC124217; MGC124218;
Gene ID : 208154
mRNA Refseq : NM_001037719
Protein Refseq : NP_001032808

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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01/25/2023

    Very satisfied with a buying experience! Worth it!

    04/01/2022

      The staff actively communicated with me and solved many technical problems.

      03/28/2022

        The BTLA proteins purchased from your company is suitable for ELISA.

        Q&As (4)

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        What are the effects of inhibiting BTLA expression? 11/02/2022

        Inhibition of BTLA expression leads to disruption of autoimmune homeostasis in the organism.

        Where is BTLA located? 07/27/2022

        BTLA is localized in the cell membrane.

        What organs are BTLA expressed in? 01/25/2022

        BTLA is widely expressed in lymphocytes of the bone marrow, thymus and spleen.

        What is the biological function of BTLA? 12/30/2021

        BTLA regulates T and B lymphocyte expression and plays a negative regulatory role in the activation phase of T lymphocytes.

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