Recombinant Mouse C4b protein, His-tagged

• Cat.No.: C4b-6785M
• Product Overview: Recombinant Mouse C4b aa. (Asn678-Arg753 (Accession # P01029)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 10.24kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Asn678-Arg753 (Accession # P01029)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >97%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Store at 4°C for short time storage (1-2 weeks). Aliquot and store at -20°C or -80°C for long term storage. Avoid repeated freeze/thaw cycles.
  12 months stored at -80°C.
• Storage buffer: Supplied as lyophilized form in PBS, pH 7.4.
• Reconstitution: Reconstitute in PBS.

Gene Information

• Gene Name: C4b complement component 4B (Chido blood group) [ Mus musculus (house mouse) ]
• Official Symbol: C4b
• Synonyms: C4; Ss; complement component 4 (within H-2S); complement component 4B (Childo blood group); complement C4-B
• Gene ID: 12268
• mRNA Refseq: NM_009780.2
• Protein Refseq: NP_033910.2
• UniProt ID: P01029

Reviews

09/05/2021

C4B labeling works very well and can be fluorescently labeled, etc., which is very suitable for our research.

02/04/2021

In experiments, biocompatibility showed up very well.

10/28/2020

Very good at western-blot experimenting.

Q&As

What are the clinical applications of C4B inhibitors?

C4B inhibitors can be used to treat diseases associated with complement hyperactivation, such as autoimmune diseases and graft-versus-host disease. By inhibiting the activity of C4B, it is possible to attenuate the excessive inflammatory response and tissue damage.

How is the expression level of C4B regulated?

The expression level of C4B is regulated by a variety of factors, including inflammatory factors, cytokines, and drugs. These factors can affect the synthesis and release of C4B, thus affecting its function in the immune system.

Is C4B involved in the formation and clearance of immune complexes?

It can bind to other complement components and immunoglobulins to form immune complexes. These immune complexes can be recognized and cleared by clearance mechanisms in the body, such as phagocytes.

Is there a relationship between C4B and other immune system components?

It is closely related to other immune system components (such as T cells, B cells, antibodies, etc.) and is involved in the immune defense response in the body. The interactions and influences between them are important for maintaining immune homeostasis.

How does C4B function differently in different cell types?

The role of C4B in different types of cells may vary. For example, in immune cells, it can be involved in the regulation of immune responses and cell activation, while in endothelial cells, it may be involved in the process of vascular inflammation and injury.

How does C4B interact with other complement components?

C4B interacts with other complement components (e.g., C4A, C2, C3, etc.) to form part of the complement activation pathway and is involved in the immune defense response.