Recombinant Mouse Car9 Protein, Myc/DDK-tagged

• Cat.No.: Car9-1962M
• Product Overview: Purified recombinant protein of mouse full-length carbonic anhydrase 9 (Car9), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Reversible hydration of carbon dioxide. Participates in pH regulation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 47.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Car9 carbonic anhydrase 9 [ Mus musculus (house mouse) ]
• Official Symbol: Car9
• Synonyms: CAR9; carbonic anhydrase 9; CA-IX; carbonic anhydrase IX; carbonate dehydratase IX; membrane antigen MN homolog; Ca9; CAIX; MN/CA9
• Gene ID: 230099
• mRNA Refseq: NM_139305
• Protein Refseq: NP_647466
• UniProt ID: Q8VHB5

Reviews

08/04/2022

Exceptional consistency, vital for our research's reliability.

12/16/2021

Critical support for our protein analysis and quantification.

02/07/2021

Unmatched accuracy in protein identification and sequencing.

Q&As

What signaling pathways are known to regulate CA9 expression?

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment?

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions?

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis?

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9?

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics?

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology?

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.