Recombinant Human CA9 cell lysate

• Cat.No.: CA9-3065HCL
• Product Overview: Human CAIX / CA9 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CA9 carbonic anhydrase IX [ Homo sapiens ]
• Official Symbol: CA9
• Synonyms: CA9; carbonic anhydrase IX; carbonic anhydrase 9; CAIX; carbonic dehydratase; MN; RCC associated protein G250; pMW1; CA-IX; P54/58N; membrane antigen MN; carbonate dehydratase IX; RCC-associated antigen G250; RCC-associated protein G250; renal cell carcinoma-associated antigen G250
• Gene ID: 768
• mRNA Refseq: NM_001216
• Protein Refseq: NP_001207
• MIM: 603179
• UniProt ID: Q16790
• Chromosome Location: 9p12
• Pathway: HIF-1-alpha transcription factor network, organism-specific biosystem
• Function: carbonate dehydratase activity; lyase activity; metal ion binding; zinc ion binding

Reviews

08/04/2022

Exceptional consistency, vital for our research's reliability.

12/16/2021

Critical support for our protein analysis and quantification.

02/07/2021

Unmatched accuracy in protein identification and sequencing.

Q&As

What signaling pathways are known to regulate CA9 expression?

The HIF-1 signaling pathway primarily regulates CA9 in response to cellular oxygen levels.

How does CA9 contribute to pH regulation within the tumor microenvironment?

CA9 aids in extracellular acidification, promoting tumor cell survival and invasion in acidic tumor microenvironments.

How is CA9 expression regulated in hypoxic conditions?

CA9 expression is upregulated by the hypoxia-inducible factor (HIF) under low oxygen conditions.

How are elevated levels of CA9 associated with cancer prognosis?

Elevated CA9 levels often correlate with poor prognosis in various cancers due to its role in tumor adaptation to hypoxia.

What post-translational modifications have been identified on CA9?

Potential modifications like glycosylation can be identified using techniques like mass spectrometry.

Are there specific inhibitors that target CA9, especially in cancer therapeutics?

Several inhibitors, like acetazolamide derivatives, have been explored to target CA9 in cancer treatment.

What roles does CA9 play in tumor physiology?

CA9 helps tumors survive in hypoxic conditions by regulating pH through the conversion of CO2 to bicarbonate and protons.