Recombinant Mouse Casp3 protein, His-tagged

• Cat.No.: Casp3-623M
• Product Overview: Recombinant Mouse Casp3 aa. (Ser29~Asp175) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Ser29~Asp175
• Description: This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants.
• Form: Freeze-dried powder
• Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: Casp3 caspase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Casp3
• Synonyms: Casp3; caspase 3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik; caspase-3; SREBP cleavage activity 1; apopain; caspase 3, apoptosis related cysteine protease; cysteine protease CPP32; protein Yama
• Gene ID: 12367
• mRNA Refseq: NM_001284409.1
• Protein Refseq: NP_001271338.1
• UniProt ID: P70677