Recombinant Mouse Casp3 Protein, MYC/DDK-tagged

• Cat.No.: Casp3-771M
• Product Overview: Purified recombinant protein of full-length mouse caspase 3 (Casp3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 31.9 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Casp3 caspase 3 [ Mus musculus (house mouse) ]
• Official Symbol: Casp3
• Synonyms: Casp3; caspase 3; CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik; caspase-3; SREBP cleavage; activity 1; apopain; caspase 3, apoptosis related cysteine protease; cysteine protease CPP32; protein Yama; EC 3.4.22.56
• Gene ID: 12367
• mRNA Refseq: NM_009810
• Protein Refseq: NP_033940
• UniProt ID: P70677

Reviews

03/29/2024

Casp3-748R, the rat caspase3 worked/is working very well for us.