Active Recombinant Mouse CD274 protein, Fc-Avi-tagged, Biotinylated

• Cat.No.: CD274-1285M
• Product Overview: Biotinylated Recombinant Mouse CD274 protein(Phe 19 - Thr 238), fused with Fc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: Fc-Avi
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
• Bio-activity: Immobilized Biotinylated Mouse CD274, Fc,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate, can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 5-39 ng/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag)The protein has a calculated MW of 53.2 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Phe 19 - Thr 238
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.