Active Recombinant Mouse Cd274 protein, hFc-tagged, Low Endotoxin

• Cat.No.: CD274-4632M
• Product Overview: Recombinant Mouse Cd274 protein(NP_068693)(19-238 aa), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Protein Length: 19-238 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Human PD-1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-L1, hFc Tag with an affinity constant of 23.90 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd274 CD274 antigen [ Mus musculus ]
• Official Symbol: Cd274
• Synonyms: CD274; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik
• Gene ID: 60533
• mRNA Refseq: NM_021893
• Protein Refseq: NP_068693