Recombinant Mouse CD36 cell lysate

• Cat.No.: CD36-2400MCL
• Product Overview: Mouse CD36 / GP3B / SCARB3 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Cd36 CD36 antigen [ Mus musculus ]
• Official Symbol: CD36
• Synonyms: CD36; CD36 antigen; platelet glycoprotein 4; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV; FAT; GPIV; Scarb3
• Gene ID: 12491
• mRNA Refseq: NM_001159555
• Protein Refseq: NP_001153027
• Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem
• Function: Gram-negative bacterial cell surface binding; Gram-positive bacterial cell surface binding; high-density lipoprotein particle binding; lipid binding; lipid binding; lipoprotein particle binding; lipoteichoic acid receptor activity; low-density lipoprotein particle binding; low-density lipoprotein particle binding; low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; receptor activity; thrombospondin receptor activity; transforming growth factor beta binding

Reviews

05/21/2020

Dependable protein purification, consistently high-quality results.

04/10/2019

Great antibody validation, enhances our immunoprecipitation experiments.

12/28/2018

Swift protein expression optimization, invaluable for our research efficiency.

Q&As

What is the impact of CD36 on inflammation and the immune response, particularly in chronic inflammatory diseases?

CD36 is involved in inflammation and immune responses, potentially exacerbating chronic inflammatory conditions.

How do alterations in CD36 expression or activity affect obesity and related health complications?

Variations in CD36 function can influence the risk of obesity and associated health issues like metabolic syndrome.

What are the mechanisms by which CD36 modulates cell signaling and energy homeostasis?

CD36 modulates cell signaling related to energy utilization and storage, impacting metabolic health.

How does CD36 contribute to the pathogenesis of metabolic disorders, such as insulin resistance and type 2 diabetes?

CD36 affects the development of metabolic disorders by influencing lipid metabolism and insulin sensitivity.

How does CD36 influence taste perception, especially in the detection of dietary fats?

CD36 aids in taste perception, particularly in detecting and responding to dietary fats.

What role does CD36 play in the development of cardiovascular diseases like atherosclerosis?

CD36 is implicated in cardiovascular diseases, contributing to the formation of atherosclerotic plaques.

How does CD36 (Cluster of Differentiation 36) function in fatty acid metabolism and uptake?

CD36 facilitates the uptake and metabolism of fatty acids in cells, playing a critical role in lipid processing.