Species : |
Mouse |
Source : |
HEK293 |
Tag : |
Fc&His |
Protein Length : |
30-439 a.a. |
Form : |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
Measured by its binding ability in a functional ELISA. Immobilized human RSPO1 at 20 μg/ml (100 μl/well) can bind mouse CD36 Fc chimera with a linear range of 6.4-800 ng/ml. |
Molecular Mass : |
The recombinant mouse CD36/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 658 amino acids and has a calculated molecular mass of 74.5 kDa. As a result of glycosylation, the apparent molecular mass of rmCD36/Fc monomer is approximately 110-120 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 88 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |