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Recombinant Mouse Cd36 protein, His-tagged

Cat.No.: Cd36-3323M
Product Overview: Recombinant Mouse Cd36(Gly 30-Lys 439) fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus was expressed in HEK293.
Description: The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 36 (CD36), also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb), is a member of the CD system as well as the class B scavenger receptor family of cell surface proteins. CD36 can be found on the surface of many cell types in vertebrate animals and it consists of 472 amino acids and is extensively glycosylated. It is an integral membrane protein primarily serving as receptors for thrombospondin and collagen and by the erythrocytes infected with the human malaria parasite. The role of CD36 as a cell surface receptor has been extended to that of a signal transduction molecule.
Source: HEK293
Species: Mouse
Tag: His
Predicted N Terminal: Gly 30
Form: Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human RSPO1-His at 10 μg/mL (100 μl/well) can bind biotinylated mouse CD36-His, The EC50 of can bind biotinylated mouse CD36-His is 0.1-0.4 μg/mL.
Molecular Mass: The secreted recombinant mouse CD36 consists of 421 amino acids and has a calculated molecular mass of 47.8 kDa. As a result of glycosylation, the apparent molecular mass of rmCD36 is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
Protein length: Gly 30-Lys 439
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Purity: > 92 % as determined by SDS-PAGE
Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name: Cd36 CD36 antigen [ Mus musculus ]
Official Symbol: Cd36
Synonyms: CD36; CD36 antigen; platelet glycoprotein 4; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV; FAT; GPIV; Scarb3;
Gene ID: 12491
mRNA Refseq: NM_001159558
Protein Refseq: NP_001153030
MIM:
UniProt ID: Q08857
Chromosome Location: 5 A3; 5 8.11 cM
Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; ECM-receptor interaction, organism-specific biosystem; ECM-receptor interaction, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem;
Function: Gram-negative bacterial cell surface binding; Gram-positive bacterial cell surface binding; high-density lipoprotein particle binding; lipid binding; lipid binding; lipoprotein particle binding; lipoteichoic acid receptor activity; low-density lipoprotein particle binding; low-density lipoprotein particle binding; low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; receptor activity; thrombospondin receptor activity; transforming growth factor beta binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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