"CD3D/CD3E" Related Products

Recombinant Mouse CD3D & CD3E Protein, Flag & His-tagged

Cat.No.: CD3D & CD3E-1251M
Product Overview: Recombinant extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at t
Source: HEK293
Species: Mouse
Tag: Flag & His
Predicted N Terminal: Phe 22 & Asp 22
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant heterodimer of mouse CD3D/CD3E comprises 254 (126+128) amino acids and has a calculated molecular mass of 29.2 (14.3+14.9) KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 20 and 22 KDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: (42.3+45.3) % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Cd3d CD3 antigen, delta polypeptide [ Mus musculus ]
Official Symbol: CD3D
Synonyms: CD3D; CD3 antigen, delta polypeptide; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3d;
Gene ID: 12500
mRNA Refseq: NM_013487
Protein Refseq: NP_038515
UniProt ID:
Pathway: Adaptive Immune System, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Dow
Function: protein binding; protein heterodimerization activity; receptor activity; transmembrane signaling receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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