Recombinant Mouse CD3D & CD3E Protein, His-Fc & Flag-Fc-tagged

• Cat.No.: CD3D & CD3E-1246M
• Product Overview: Recombinant extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646)

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&Flag&His
• Protein Length: 1-105;1-108 a.a.
• Predicted N Terminal: Phe 22 & Asp 22
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant heterodimer of mouse CD3D/CD3E comprises 670 (333+337) amino acids and has a calculated molecular mass of 76 (38+38) kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cd3d CD3 antigen, delta polypeptide [ Mus musculus ]
• Official Symbol: CD3D
• Synonyms: CD3D; CD3 antigen, delta polypeptide; T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; T3d
• Gene ID: 12500
• mRNA Refseq: NM_013487
• Protein Refseq: NP_038515
• Pathway: Adaptive Immune System, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Dow
• Function: protein binding; protein heterodimerization activity; receptor activity; transmembrane signaling receptor activity