Recombinant Mouse CD3G Protein


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Recombinant Mouse CD3G Protein

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Cat.No. : Cd3g-324M
Product Overview : Recombinant extracellular domain (Met1-Asp108) of mouse CD3E (NP_031674.1) was fused with the C-terminal polyhistidine tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of mouse CD3G (NP_033980.1) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3E/CD3G heterodimer was purified.
Source : HEK293
Species : Mouse
Predicted N Terminal : Asp 23 & Gln 23
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant heterodimer of mouse CD3E/CD3G comprises 671 (336+335) amino acids and has a calculated molecular mass of 75.4 (38.1+37.3) KDa.
Protein length : Met1-Ser116
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : 95.1 % as determined by SDS-PAGE
Storage : Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Cd3g CD3 antigen, gamma polypeptide [ Mus musculus ]
Official Symbol : Cd3g
Synonyms : CD3G; CD3 antigen, gamma polypeptide; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; T3g; Ctg3; Ctg-3;
Gene ID : 12502
mRNA Refseq : NM_009850
Protein Refseq : NP_033980

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