Recombinant Mouse CD3G Protein

• Cat.No.: Cd3g-324M
• Product Overview: Recombinant extracellular domain (Met1-Asp108) of mouse CD3E (NP_031674.1) was fused with the C-terminal polyhistidine tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Ser116) of mouse CD3G (NP_033980.1) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3E/CD3G heterodimer was purified.

Specification

• Source: HEK293
• Species: Mouse
• Predicted N Terminal: Asp 23 & Gln 23
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant heterodimer of mouse CD3E/CD3G comprises 671 (336+335) amino acids and has a calculated molecular mass of 75.4 (38.1+37.3) KDa.
• Protein length: Met1-Ser116
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: 95.1 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Cd3g CD3 antigen, gamma polypeptide [ Mus musculus ]
• Official Symbol: Cd3g
• Synonyms: CD3G; CD3 antigen, gamma polypeptide; T-cell surface glycoprotein CD3 gamma chain; T-cell receptor T3 gamma chain; T3g; Ctg3; Ctg-3
• Gene ID: 12502
• mRNA Refseq: NM_009850
• Protein Refseq: NP_033980