Active Recombinant Mouse Cd40lg protein, monomeric hFc-tagged, Low Endotoxin

• Cat.No.: Cd40lg-7854M
• Product Overview: Recombinant Mouse Cd40lg protein(P27548)(112-260 aa), fused with N-terminal monomeric hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Protein Length: 112-260 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse CD40, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Mouse CD40 Ligand Trimer, hFc Tag with the EC50 of 3.5 ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 75.6 kDa. Due to glycosylation, the protein migrates to 76-80 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Cd40lg CD40 ligand [ Mus musculus ]
• Official Symbol: Cd40lg
• Synonyms: CD40LG; CD40 ligand; T-cell antigen Gp39; TNF-related activation protein; tumor necrosis factor ligand superfamily member 5; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5
• Gene ID: 21947
• mRNA Refseq: NM_011616
• Protein Refseq: NP_035746