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Recombinant Mouse CD44 Protein (Met1-Thr224), His-tagged

Cat.No. : CD44-891M
Product Overview : Recombinant Mouse CD44 Protein (NP_001034240.1) (Met1-Thr224) was produced by HEK293 Cells expression system. This protein was expressed with a polyhistidine tag at the C-terminus.
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Description : CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Gln 25
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant mouse CD44 consists of 211 amino acids and predicts a molecular mass of 23.6 kDa.
Protein length : Met1-Thr224
Endotoxin : < 1.0 EU per μg protein as determined by the LAL method.
Purity : > 95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : Cd44 CD44 antigen [ Mus musculus ]
Official Symbol : CD44
Synonyms : CD44; CD44 antigen; PGP-I; HUTCH-I; ECMR-III; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109;
Gene ID : 12505
mRNA Refseq : NM_001039150
Protein Refseq : NP_001034239
UniProt ID : Q3U8S1

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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What is the role of CD44 in cancer? 04/27/2023

CD44 is implicated in cancer progression and metastasis. It promotes tumor cell invasion, migration, and adhesion to the extracellular matrix. CD44 also plays a role in cancer stem cell maintenance and drug resistance. Its overexpression is associated with poor prognosis in various types of cancer.

 Can CD44 be targeted for therapeutic purposes? 05/19/2021

Targeting CD44 has been explored as a potential therapeutic strategy in cancer and inflammatory diseases. Strategies include blocking CD44-ligand interactions, inhibiting CD44 signaling pathways, and targeting CD44-positive cancer stem cells. However, further research is needed to fully understand the complex roles of CD44 in different disease contexts and to develop effective CD44-targeted therapies.

How is the expression of CD44 regulated? 03/10/2021

The expression of CD44 is regulated at multiple levels, including transcriptional control, alternative splicing, and post-translational modifications. Transcription factors such as Sp1 and NF-κB can bind to the CD44 promoter region to regulate its gene expression. Additionally, alternative splicing of CD44 pre-mRNA generates CD44 isoforms with different functions and expression patterns.

 How does CD44 contribute to immune regulation? 11/02/2019

CD44 is involved in immune cell activation, trafficking, and homing. It participates in the recruitment of leukocytes to sites of inflammation and mediates T cell adhesion and migration. CD44 can also modulate immune responses by interacting with other immune receptors and cytokines, and it is involved in the regulation of T cell activation and differentiation.

How is CD44 involved in immune regulation? 05/12/2019

CD44 plays a vital role in immune regulation by participating in immune cell activation, migration, and modulation of T-cell activation and differentiation. CD44 is expressed on various immune cell populations, including T cells, B cells, natural killer cells, and dendritic cells. It serves as an adhesion molecule and facilitates immune cell trafficking to lymphoid tissues and inflammatory sites. CD44 also functions as a co-stimulatory receptor, enhancing T-cell activation and cytokine production upon interaction with its ligands. Moreover, CD44 isoforms have been implicated in the differentiation of T helper cell subsets, such as Th1, Th2, and Th17 cells. These findings highlight the multifaceted role of CD44 in immune regulation and its potential as a therapeutic target for immune-related disorders.

What is the cellular localization of the protein CD44? 02/11/2016

CD44 is a transmembrane glycoprotein predominantly localized on the cell surface, specifically in the plasma membrane of various cell types, including epithelial cells, leukocytes, and mesenchymal cells.

 What are the signaling pathways associated with CD44? 01/03/2016

CD44 can activate various signaling pathways, including PI3K/AKT, MAPK/ERK, and NF-κB pathways. These pathways mediate downstream cellular processes such as cell survival, proliferation, migration, and immune responses. CD44 can also interact with other cell surface receptors, such as receptor tyrosine kinases, to modulate their signaling activities.

Customer Reviews (3)

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Reviews
03/08/2022

    Using this reagent, I can gain a better understanding of the functionality and regulatory mechanisms of proteins.

    04/26/2019

      This reagent has long-lasting performance, enduring prolonged experimental use.

      03/14/2019

        A miraculous reagent driving us towards the frontiers, inviting a glance into an expanded world.

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