Recombinant Mouse CD6 cell lysate

• Cat.No.: CD6-1807MCL
• Product Overview: Mouse CD6 / TP120 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Cd6 CD6 antigen [ Mus musculus ]
• Official Symbol: CD6
• Synonyms: CD6; CD6 antigen; T-cell differentiation antigen CD6
• Gene ID: 12511
• mRNA Refseq: NM_001037801
• Protein Refseq: NP_001032890
• Pathway: Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem
• Function: glycoprotein binding; protein binding; protein kinase binding; scavenger receptor activity

Reviews

04/05/2021

Fantastic customer support. They genuinely care about the success of our research.

11/11/2020

Quality you can trust. Our lab has seen improved precision since using this product.

07/27/2019

Shipping is always on time, and the packaging ensures the product arrives in perfect condition.

Q&As

Can you explain the role of CD6 in T-cell co-stimulation and its implications for adaptive immune responses?

CD6 provides co-stimulatory signals to T cells, influencing their activation and differentiation. This contributes to the regulation of adaptive immune responses.

How are CD6 genes polymorphic, and what is the significance of genetic variations in the context of autoimmune diseases?

CD6 genes exhibit polymorphisms, and certain variations are associated with susceptibility to autoimmune diseases, indicating a potential role in immune dysregulation.

How does CD6 contribute to the formation of immunological synapses, and what molecular events are involved in this process?

CD6 is involved in immunological synapse formation, and its interactions with ligands, such as ALCAM, play a role in stabilizing T-cell interactions with antigen-presenting cells.

How does CD6 influence T-cell trafficking and migration, and what are the implications for immune surveillance and inflammation?

CD6 modulates T-cell trafficking and migration, impacting immune surveillance and inflammation. Its interactions with adhesion molecules contribute to these processes.

What is the relationship between CD6 and antigen-presenting cells, and how does it impact the initiation of immune responses?

CD6 interacts with antigen-presenting cells, facilitating T-cell activation. This interaction is pivotal for the initiation and regulation of immune responses.