Recombinant Mouse Cdkn1a Protein, MYC/DDK-tagged

• Cat.No.: Cdkn1a-869M
• Product Overview: Purified recombinant protein of full-length mouse cyclin-dependent kinase inhibitor 1A (P21) (Cdkn1a), with C-terminal MYC/DDK tag, was expressed in HEK293T cells.

Specification

• Description: This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at the G1 pahse. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Alternative splicing results in multiple transcript variants.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 17.8 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 µg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cdkn1a cyclin-dependent kinase inhibitor 1A (P21) [ Mus musculus (house mouse) ]
• Official Symbol: Cdkn1a
• Synonyms: Cdkn1a; cyclin-dependent kinase inhibitor 1A (P21); P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP20; Cdkn1; p21WAF; p21Cip1; cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; melanoma differentiation-associated protein
• Gene ID: 12575
• mRNA Refseq: NM_001111099
• Protein Refseq: NP_001104569
• UniProt ID: P39689