Recombinant Mouse Chst5 Protein, His-tagged

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Recombinant Mouse Chst5 Protein, His-tagged

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Cat.No. : Chst5-06M
Product Overview : Recombinant mouse CHST5 (27-395aa), fused to His-tag at C terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description : The protein encoded by this gene belongs to the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family, members of which catalyze the transfer of sulfate to position 6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or N-acetylglucosamine (GlcNAc) residues within proteoglycans, and sulfation of O-linked sugars of mucin-type acceptors. Carbohydrate sulfation plays a critical role in many biologic processes. This gene is predominantly expressed in colon and small intestine.
Source : Insects cells
Species : Mouse
Tag : His
Form : Liquid
Bio-activity : Specific activity is > 10,000 pmol/min/μg, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37 centigrade.
Molecular Mass : 42.9kDa (380aa)
AA Sequence : <ADPEF>SRQ VPSSPAGLGE RVHVLVLSSW RSGSSFVGQL FSQHPDVFYL MEPAWHVWDT LSQGSAPALH MAVRDLIRSV FLCDMDVFDA YLPWRRNISD LFQWAVSRAL CSPPVCEAFA RGNISSEEVC KPLCATRPFG LAQEACSSYS HVVLKEVRFF NLQVLYPLLS DPALNLRIVH LVRDPRAVLR SREQTAKALA RDNGIVLGTN GTWVEADPRL RVVNEVCRSH VRIAEAALHK PPPFLQDRYR LVRYEDLARD PLTVIRELYA FTGLGLTPQL QTWIHNITHG SGPGARREAF KTTSRDALSV SQAWRHTLPF AKIRRVQELC GGALQLLGYR SVHSELEQRD LSLDLLLPRG MDSFKWASST EKQPES<HHH HHH>
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Enzyme Activity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.25 mg/mL (determined by Bradford assay)
Storage Buffer : In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Gene Name : Chst5 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 [ Mus musculus (house mouse) ]
Official Symbol : Chst5
Synonyms : Chst5; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5; GST-; GST-4; I-GlcN; Gn6st-3; AI173964; I-GlcNAc-6-ST; carbohydrate sulfotransferase 5; GST4; I-GlcNAc6ST; N-acetylglucosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4; glcNAc6ST-3; intestinal GlcNAc-6-sulfotransferase; intestinal N-acetylglucosamine-6-O-sulfotransferase; mIGn6ST; EC 2.8.2.-
Gene ID : 56773
mRNA Refseq : NM_019950
Protein Refseq : NP_064334
UniProt ID : Q9QUP4
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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