Recombinant Mouse Chst5 Protein, His-tagged
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Cat.No. : | Chst5-06M Optional Service: Optional requirements on this protein |
Product Overview : | Recombinant mouse CHST5 (27-395aa), fused to His-tag at C terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
Description : | The protein encoded by this gene belongs to the Gal/GalNAc/GlcNAc 6-O-sulfotransferase (GST) family, members of which catalyze the transfer of sulfate to position 6 of galactose (Gal), N-acetylgalactosamine (GalNAc), or N-acetylglucosamine (GlcNAc) residues within proteoglycans, and sulfation of O-linked sugars of mucin-type acceptors. Carbohydrate sulfation plays a critical role in many biologic processes. This gene is predominantly expressed in colon and small intestine. |
Source : | Insects cells |
Species : | Mouse |
Tag : | His |
Form : | Liquid |
Bio-activity : | Specific activity is > 10,000 pmol/min/μg, and is defined as the amount of enzyme that sulfate from PAPS to N-acetyl-D-glucosamine per minute at pH 7.5, at 37 centigrade. |
Molecular Mass : | 42.9kDa (380aa) |
AA Sequence : | <ADPEF>SRQ VPSSPAGLGE RVHVLVLSSW RSGSSFVGQL FSQHPDVFYL MEPAWHVWDT LSQGSAPALH MAVRDLIRSV FLCDMDVFDA YLPWRRNISD LFQWAVSRAL CSPPVCEAFA RGNISSEEVC KPLCATRPFG LAQEACSSYS HVVLKEVRFF NLQVLYPLLS DPALNLRIVH LVRDPRAVLR SREQTAKALA RDNGIVLGTN GTWVEADPRL RVVNEVCRSH VRIAEAALHK PPPFLQDRYR LVRYEDLARD PLTVIRELYA FTGLGLTPQL QTWIHNITHG SGPGARREAF KTTSRDALSV SQAWRHTLPF AKIRRVQELC GGALQLLGYR SVHSELEQRD LSLDLLLPRG MDSFKWASST EKQPES<HHH HHH> |
Endotoxin : | < 1 EU/μg of protein (determined by LAL method) |
Purity : | > 90% by SDS-PAGE |
Applications : | SDS-PAGE, Enzyme Activity |
Storage : | Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 0.25 mg/mL (determined by Bradford assay) |
Storage Buffer : | In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol |
SDS-PAGE : |
3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain. |
Gene Name : | Chst5 carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5 [ Mus musculus (house mouse) ] |
Official Symbol : | Chst5 |
Synonyms : | Chst5; carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 5; GST-; GST-4; I-GlcN; Gn6st-3; AI173964; I-GlcNAc-6-ST; carbohydrate sulfotransferase 5; GST4; I-GlcNAc6ST; N-acetylglucosamine 6-O-sulfotransferase 3; galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 4; glcNAc6ST-3; intestinal GlcNAc-6-sulfotransferase; intestinal N-acetylglucosamine-6-O-sulfotransferase; mIGn6ST; EC 2.8.2.- |
Gene ID : | 56773 |
mRNA Refseq : | NM_019950 |
Protein Refseq : | NP_064334 |
UniProt ID : | Q9QUP4 |
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