Recombinant Mouse Cldn3 protein, His-tagged Cldn3-1427M

Recombinant Mouse Cldn3 protein, His-tagged

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Recombinant Mouse Cldn3 protein, His-tagged

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Cat.No. : Cldn3-1427M
Product Overview : Recombinant Mouse Cldn3 aa. (Arg30~Met159 (Accession # Q9Z0G9) fused with N-terminal His tag was produced in E. coli cells.
Description : This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a low-affinity receptor for clostridium perfringens enterotoxin (CPE) produced by the bacterium Clostridium perfringens, and the interaction with CPE results in increased membrane permeability by forming small pores in plasma membrane. This protein is highly overexpressed in uterine carcinosarcoma. This protein is also predominantly present in brain endothelial cells, where it plays a specific role in the establishment and maintenance of blood brain barrier tight junction morphology.
Source : E. coli
Species : Mouse
Tag : His
Form : Freeze-dried powder
Molecular Mass : 16kDa as determined by SDS-PAGE reducing conditions.
Protein length : Arg30~Met159 (Accession # Q9Z0G9)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 6.6.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Gene Name : Cldn3 claudin 3 [ Mus musculus (house mouse) ]
Official Symbol : Cldn3
Synonyms : Cldn3; claudin 3; mRVP1; Cpetr2; AI182374; claudin-3; CPE-R 2; CPE-receptor 2; clostridium perfringens enterotoxin receptor 2
Gene ID : 12739
mRNA Refseq : NM_009902.4
Protein Refseq : NP_034032.1
UniProt ID : Q9Z0G9

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