"CNTF" Related Products

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Recombinant Mouse CNTF Protein, His-tagged

Cat.No.: CNTF-995M
Product Overview: Recombinant mouse CNTF (NP_740756.1) (Ala2-Met198) was expressed with a polyhistidine tag at the N-terminus.
Source: E. coli
Species: Mouse
Tag: His
Predicted N Terminal: His
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its binding ability in a functional ELISA. Immobilized mouse CNTF at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His. The EC50 of biotinylated rat CNTFR-His is 5-12 ng/ml.2. Measured in a cell proliferation assay using TF-1 human
Molecular Mass: The recombinant mouse CNTF consists of 215 amino acids and predicts a molecular mass of 24.7 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Cntf ciliary neurotrophic factor [ Mus musculus ]
Official Symbol: CNTF
Synonyms: CNTF; ciliary neurotrophic factor; AI429687; MGC41235;
Gene ID: 12803
mRNA Refseq: NM_170786
Protein Refseq: NP_740756
MIM:
UniProt ID:
Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT
Function: ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; interleukin-6 receptor binding;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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