Recombinant Mouse Copb1 Protein, Myc/DDK-tagged

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Recombinant Mouse Copb1 Protein, Myc/DDK-tagged

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Cat.No. : Copb1-2257M
Product Overview : Purified recombinant protein of mouse full-length coatomer protein complex, subunit beta 1 (Copb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
Description : The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins in cortical neurons. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 107.5 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Copb1 coatomer protein complex, subunit beta 1 [ Mus musculus (house mouse) ]
Official Symbol : Copb1
Synonyms : COPB1; coatomer protein complex, subunit beta 1; coatomer subunit beta; beta-COP; beta-coat protein; 2610019B04Rik
Gene ID : 70349
mRNA Refseq : NM_033370
Protein Refseq : NP_203534
UniProt ID : Q9JIF7

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