Active Recombinant Mouse Csf1r Protein, His-Avi-tagged, Biotinylated

• Cat.No.: Csf1r-4001M
• Product Overview: Biotinylated Mouse M-CSF R, His Tag is expressed from HEK293 cells. It contains AA Ala 20 - Ser 511 (Accession # P09581-1). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: 20-511 a.a.
• Predicted N Terminal: Ala 20
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4 , 10% trehalose.
• Bio-activity: Immobilized Human M-CSF, His Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Mouse M-CSF R, His Tag with a linear range of 0.625-10 ug/mL.
• Molecular Mass: The protein has a calculated MW of 58.4 kDa. The protein migrates as 78-115 kDa under reducing condition (SDS-PAGE) due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: Csf1r colony stimulating factor 1 receptor [ Mus musculus ]
• Official Symbol: Csf1r
• Synonyms: CSF1R; colony stimulating factor 1 receptor; macrophage colony-stimulating factor 1 receptor; c-fms; CSF-1-R; CSF-1 receptor; proto-oncogene fms; proto-oncogene c-Fms; Fms; CD115; Csfmr; Fim-2; CSF-1R; M-CSFR; M-CSF-R; AI323359
• Gene ID: 12978
• mRNA Refseq: NM_001037859
• Protein Refseq: NP_001032948